As these vials are no longer produced, Genencor International – DuPont started testing Soleris vials in order to decide whether these can be a trustworthy alternative in the future. Until recently the latter analysis was performed by using BioLumix vials in Genencor International - DuPont plants. This can be tested in two ways: by carrying out a manual analysis on so-called ‘plates’ or by exercising an analysis by means of vials in a BioLumix machine. It is extremely important that the final product, the enzymes, are completely uncontaminated by these microorganisms. In Genencor International - DuPont Bruges, three kinds of microorganisms are used to produce enzymes: bacteria, yeast and molds. The calibration curves of the remaining enzyme types also need to be obtained and validated.Ībstract Bachelor Project -2019: Rapid, automatic and innovative micro biology after validation of end products by means of Soleris vials in a BioLumix machineĮnzymes act as catalysts in many biochemical processes. In the near future, the already existing calibration curves of the analysed enzymes need to be updated with measurements only made with reflector type B. Both of these are above the established maximum limit.Īlthough the FOSS NIRS DS2500 is not fully ready to be implemented in the laboratory, the prospects are very promising. The obtained average percentage difference with this reflector was of 6,10 %, and of 6,96 % with reflector A. The use of different reflectors have shown to influence the measurements of enzyme activity, with reflector B showing slightly better results. With the older spectrometer an average of 5,24 % is obtained. The percentage difference is lower with the DS2500, with an average of 3,91 %. Overall, the FOSS NIRS DS2500 showed an improvement over the FOSS NIR 5000 DCA. The study showed that the results were desirable with two out of the three analysed types of enzymes, as the average percentage difference between the enzyme activity measurements and the reference values is in both cases below the maximum established limit of 5 %. Two other conditions were simultaneously studied: whether the FOSS NIRS DS2500 shows a noticeable improvement over the spectrometer that is due replacement (FOSS NIR 5000 DCA) and whether the use of different types of reflectors influences the analysis. Finally, results between both methods were compared. In the second step, following the integration of the calibration curves in the software of the spectrometer, enzyme samples were measured with both the near-infrared method and an autoanalyzer colorimetric method. The company then made a calibration curve for each type of analysed enzyme. In the first step, a minimum of hundred measurements were carried out of each type of enzyme and the resulting spectra were collected and sent to the manufacturing company of the spectrometer. As to apply this spectrometer in the daily laboratory routine, it needs to be validated. Therefore, a new spectrometer, the FOSS NIRS DS2500, has been recently purchased as a replacement. The previously acquired near-infrared spectrometer is no longer supported by its manufacturer. In the need of a faster and cheaper analysis, near infrared spectroscopy was implemented for the enzyme activity measurement of fermentation samples. Traditional techniques, such as manual or automated colorimetric analysis, require sample and reagent preparation and thus are time-consuming and demanding methods. The determination of enzyme activity is a key analysis in the monitoring of the enzyme production process. Abstract Bachelor Project FBT 2019-2020: Validation of the calibration curves from enzyme activity obtained with NIR-spectrometry by means of an autoanalyzer
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |